4Q2T
Crystal structure of Arginyl-tRNA synthetase complexed with L-arginine
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 5C (4A) |
Synchrotron site | PAL/PLS |
Beamline | 5C (4A) |
Detector technology | CCD |
Collection date | 2013-05-03 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97949 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 74.485, 106.901, 175.248 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 38.910 - 2.400 |
R-factor | 0.1981 |
Rwork | 0.196 |
R-free | 0.25760 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1bs2 |
RMSD bond length | 0.009 |
RMSD bond angle | 1.122 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.440 |
High resolution limit [Å] | 2.400 | 6.510 | 2.400 |
Rmerge | 0.075 | 0.051 | 0.306 |
Number of reflections | 55170 | ||
<I/σ(I)> | 13.5 | ||
Completeness [%] | 97.8 | 96.9 | 95.4 |
Redundancy | 4.8 | 6.1 | 3.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.7 | 293 | 0.085mM Sodium citrate tribasic dehydrate, 24% PEG4000, 0.17M Ammonium acetate, 15% Glycerol, pH 5.7, VAPOR DIFFUSION, HANGING DROP, temperature 293K |