4Q2J
A novel structure-based mechanism for DNA-binding of SATB1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-03-19 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.9791 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 128.978, 91.971, 100.239 |
| Unit cell angles | 90.00, 128.97, 90.00 |
Refinement procedure
| Resolution | 42.472 - 2.603 |
| R-factor | 0.2301 |
| Rwork | 0.228 |
| R-free | 0.27070 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.309 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER (2.4) |
| Refinement software | PHENIX ((phenix.refine: 1.6.4_486)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.690 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.054 | |
| Number of reflections | 25454 | |
| <I/σ(I)> | 16.1 | |
| Completeness [%] | 72.1 | 94.8 |
| Redundancy | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 10% polyethylene glycol 3350, 0.1M sodium malonate pH 7.0, 0.2M glycine, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
