4Q2F

Galectin-1 in Complex with Ligand AN020

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Detector technology	PIXEL
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9766
Spacegroup name	P 21 21 21
Unit cell lengths	43.295, 58.411, 111.075
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.339 - 1.400
R-factor	0.1832
Rwork	0.183
R-free	0.19570
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.015
RMSD bond angle	1.787
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.8.2_1309))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	55.537
High resolution limit [Å]	1.400	1.400
Number of reflections	56022
<I/σ(I)>	15.6
Completeness [%]	99.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	289	ammonium sulfate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K