4Q28

Crystal Structure of the Plectin 1 and 2 Repeats of the Human Periplakin. Northeast Structural Genomics Consortium (NESG) Target HR9083A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X4C
Synchrotron site	NSLS
Beamline	X4C
Temperature [K]	100
Detector technology	CCD
Collection date	2014-03-13
Detector	MAR CCD 165 mm
Wavelength(s)	0.97900
Spacegroup name	P 31 2 1
Unit cell lengths	68.237, 68.237, 192.587
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	34.118 - 2.640
R-factor	0.216
Rwork	0.214
R-free	0.25700
Structure solution method	SAD
RMSD bond length	0.007
RMSD bond angle	1.330
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SHELXS
Refinement software	PHENIX (1.7.2_869)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.730
High resolution limit [Å]	2.640	2.640
Rmerge	0.114	0.857
Number of reflections	29474
<I/σ(I)>	22.5	1.5
Completeness [%]	99.9	100
Redundancy	11	8.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Microbatch crystallization under oil	9	277	Protein solution: 100mM NaCl, 5mM DTT, 0.02% NaN3, 10mM Tris-HCl (pH 7.5). Reservoir solution: 40% PEG 1000, 0.1M magnesium nitrate, 0.1M TAPS, pH 9.0, Microbatch crystallization under oil, temperature 277K