4Q26

Crystal Structure of Galectin-1 in Complex with N-Acetyllactosamine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	PIXEL
Detector	DECTRIS PILATUS 6M-F
Spacegroup name	P 21 21 2
Unit cell lengths	66.384, 85.517, 116.076
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.023 - 1.399
R-factor	0.1629
Rwork	0.162
R-free	0.20290
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.018
RMSD bond angle	2.023
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.7.1_743))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.023
High resolution limit [Å]	1.399	1.399
Number of reflections	129682
Completeness [%]	99.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	289	pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K