4Q25

Crystal structure of PhoU from Pseudomonas aeruginosa

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	100
Detector technology	CCD
Collection date	2009-04-21
Detector	ADSC QUANTUM 210
Wavelength(s)	1.0000
Spacegroup name	P 41 21 2
Unit cell lengths	70.483, 70.483, 196.203
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.890 - 2.280
R-factor	0.21041
Rwork	0.209
R-free	0.23426
Structure solution method	SAD
RMSD bond length	0.011
RMSD bond angle	1.363
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SOLVE
Refinement software	REFMAC (5.7.0029)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.320
High resolution limit [Å]	2.280	2.280
Number of reflections	22783
Completeness [%]	99.9	98.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	297	0.1M sodium malonate, pH 4.0, 12% PEG 3350, VAPOR DIFFUSION, SITTING DROP, temperature 297K