4Q24
Crystal structure of Cyclo(L-leucyl-L-phenylalanyl) synthase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-07-13 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.984 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 86.968, 86.968, 70.738 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 37.660 - 2.900 |
| R-factor | 0.2034 |
| Rwork | 0.201 |
| R-free | 0.25260 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3oqv |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.070 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.700 | 3.070 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Number of reflections | 7023 | |
| <I/σ(I)> | 11.45 | 1.75 |
| Completeness [%] | 98.1 | 98.5 |
| Redundancy | 2.71 | 2.72 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 278 | 10%PEG8000, 50mM KCl, 50mM Na cacodylate, pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 278K |
