4Q1V

Crystal structure of a putative dipeptidyl aminopeptidase IV (BACOVA_01349) from Bacteroides ovatus ATCC 8483 at 2.48 A resolution

Experimental procedure

Experimental method	SAD
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL14-1
Synchrotron site	SSRL
Beamline	BL14-1
Temperature [K]	100
Detector technology	CCD
Collection date	2013-12-16
Detector	MARMOSAIC 325 mm CCD
Spacegroup name	P 21 21 21
Unit cell lengths	92.487, 133.703, 159.127
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.304 - 2.480
R-factor	0.1731
Rwork	0.171
R-free	0.20740
Structure solution method	SAD
RMSD bond length	0.010
RMSD bond angle	1.100
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	SHELX
Refinement software	BUSTER-TNT (2.10.0)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	49.304		2.570
High resolution limit [Å]	2.480	5.310	2.480
Rmerge	0.152	0.060	0.919
Number of reflections	70624	13750	13722
<I/σ(I)>	6.84	13.8	1.7
Completeness [%]	99.7	99.6	100

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	293	10.0% polyethylene glycol 6000, 0.1M MES pH 6.0, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 293K