4Q1S
Yeast 20S proteasome in Complex with Kendomycin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2012-12-08 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 1.0 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 136.440, 301.120, 145.540 |
Unit cell angles | 90.00, 113.15, 90.00 |
Refinement procedure
Resolution | 48.525 - 2.600 |
R-factor | 0.1779 |
Rwork | 0.175 |
R-free | 0.22369 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ryp |
RMSD bond length | 0.011 |
RMSD bond angle | 1.606 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | REFMAC |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.525 | 2.700 |
High resolution limit [Å] | 2.600 | 2.600 |
Rmerge | 0.060 | 0.485 |
Number of reflections | 324230 | |
<I/σ(I)> | 14.3 | 2.7 |
Completeness [%] | 98.2 | 99.3 |
Redundancy | 3.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 20 mM MgAc2, 100 mM MES, 13% MPD, pH 6,8, VAPOR DIFFUSION, HANGING DROP, temperature 293K |