4Q1R

Galectin-1 in Complex with Ligand AN027

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	PIXEL
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9764
Spacegroup name	P 21 21 21
Unit cell lengths	43.140, 58.410, 111.170
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	51.707 - 1.470
R-factor	0.1561
Rwork	0.154
R-free	0.19390
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.011
RMSD bond angle	1.546
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.8.2_1309))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	51.707
High resolution limit [Å]	1.470	1.470
Number of reflections	48330
<I/σ(I)>	15.8
Completeness [%]	99.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	289	ammonium sulfate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K