4Q1P

Galectin-1 in Complex with Ligand NB169

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	PIXEL
Detector	DECTRIS PILATUS 6M-F
Spacegroup name	P 21 21 21
Unit cell lengths	43.267, 58.373, 111.066
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.316 - 1.460
R-factor	0.1511
Rwork	0.149
R-free	0.18820
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.013
RMSD bond angle	1.537
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.8.2_1309))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	55.533
High resolution limit [Å]	1.460	1.460
Number of reflections	49191
Completeness [%]	99.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	289	1.5 M ammonium sulfate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K