4Q11
Crystal structure of Proteus mirabilis transcriptional regulator protein Crl at 1.95A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-02-03 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97911 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 57.585, 36.195, 60.880 |
| Unit cell angles | 90.00, 115.18, 90.00 |
Refinement procedure
| Resolution | 30.250 - 1.950 |
| R-factor | 0.18961 |
| Rwork | 0.185 |
| R-free | 0.23779 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3rpj |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.401 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.250 | 2.000 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.045 | 0.629 |
| Number of reflections | 16783 | |
| <I/σ(I)> | 13.2 | 1.6 |
| Completeness [%] | 99.4 | 99.1 |
| Redundancy | 3.3 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9.5 | 291 | 100mM CHES-NaOH, PEG3000 30%(w/v), pH 9.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
