4Q10
The catalytic core of Rad2 in complex with DNA substrate (complex IV)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.2 |
Synchrotron site | BESSY |
Beamline | 14.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-05-23 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.89440 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 98.680, 97.958, 112.038 |
Unit cell angles | 90.00, 96.41, 90.00 |
Refinement procedure
Resolution | 43.133 - 2.700 |
R-factor | 0.2344 |
Rwork | 0.232 |
R-free | 0.28410 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4q0r |
RMSD bond length | 0.007 |
RMSD bond angle | 1.006 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 111.300 | 2.750 |
High resolution limit [Å] | 2.700 | 2.700 |
Rmerge | 0.071 | 0.603 |
Number of reflections | 27918 | |
<I/σ(I)> | 19.7 | 1.9 |
Completeness [%] | 95.5 | 99.9 |
Redundancy | 3.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 291 | 8% (w/v) PEG 8000, 18% (v/v) ethylene glycol, 0.2 M D-glucose, 0.2 M D-mannose, 0.2 M D-galactose, 0.2 M L-fructose, 0.2 M D-xylose, 0.2 M N-acetyl-D-glucosamine, and 0.1 M MOPS/HEPES-Na, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 291K |