4Q0X
Crystal structure of non-neutralizing antibody in complex with Epitope II of HCV E2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-07-19 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 43 2 2 |
| Unit cell lengths | 49.991, 49.991, 390.585 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.900 |
| R-factor | 0.22427 |
| Rwork | 0.221 |
| R-free | 0.28454 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.222 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 97.646 | 2.980 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Number of reflections | 12050 | |
| Completeness [%] | 99.0 | 95.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 0.1 M imidazole, 14% w/v PEG550 MME, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
