4Q0W

he catalytic core of Rad2 in complex with DNA substrate (complex II)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.2
Synchrotron site	BESSY
Beamline	14.2
Temperature [K]	100
Detector technology	CCD
Collection date	2013-05-23
Detector	RAYONIX MX-225
Wavelength(s)	0.89440
Spacegroup name	P 21 21 2
Unit cell lengths	126.641, 76.813, 108.192
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.386 - 2.100
R-factor	0.1857
Rwork	0.183
R-free	0.22990
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4q0r
RMSD bond length	0.007
RMSD bond angle	1.053
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.8.2_1309))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	2.140
High resolution limit [Å]	2.140	2.100
Rmerge	0.088	0.831
Number of reflections	62159
<I/σ(I)>		2.1
Completeness [%]	99.8	99.3
Redundancy	7.1	6.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	291	9% (w/v) PEG 20000, 20% (v/v) PEG MME 550, 0.2 M D-glucose, 0.2 M D-mannose, 0.2 M D-galactose, 0.2 M L-fructose, 0.2 M D-xylose, 0.2 M N-acetyl-D-glucosamine, and 0.1 M MOPS/HEPES-Na, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 291K