4Q0K

Crystal Structure of Phytohormone Binding Protein from Medicago truncatula in complex with gibberellic acid (GA3)

Replaces:  3US7

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.2
Synchrotron site	BESSY
Beamline	14.2
Temperature [K]	100
Detector technology	CCD
Collection date	2011-04-27
Detector	RAYONIX MX-225
Wavelength(s)	0.91800
Spacegroup name	P 65
Unit cell lengths	55.800, 55.800, 99.960
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	34.750 - 1.340
R-factor	0.1238
Rwork	0.123
R-free	0.15720
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2flh
RMSD bond length	0.019
RMSD bond angle	1.651
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.8.4_1496)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	34.750	4.070
High resolution limit [Å]	1.340	2.890
Rmerge	0.043	0.024
Number of reflections	38199	2553	1431
<I/σ(I)>	30.64	96.92
Completeness [%]	97.8	100
Redundancy	10.87

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	292	0.1 M ADA, 1.0 M AMMONIUM SULFATE, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 292K