4Q0J

Deinococcus radiodurans BphP photosensory module

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-F
Synchrotron site	APS
Beamline	21-ID-F
Temperature [K]	100
Detector technology	CCD
Collection date	2013-02-16
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.97872
Spacegroup name	P 64 2 2
Unit cell lengths	148.961, 148.961, 148.929
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	46.339 - 2.747
R-factor	0.2034
Rwork	0.202
R-free	0.22360
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.730
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.8.4_1496))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.747
Number of reflections	25912
Completeness [%]	99.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	296	18% PEG 3350, 5% tacsimate pH 6.5, 2% ethylene glycol, 100 mM PIPES pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 296K