4Q0J
Deinococcus radiodurans BphP photosensory module
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-F |
Synchrotron site | APS |
Beamline | 21-ID-F |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-02-16 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.97872 |
Spacegroup name | P 64 2 2 |
Unit cell lengths | 148.961, 148.961, 148.929 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 46.339 - 2.747 |
R-factor | 0.2034 |
Rwork | 0.202 |
R-free | 0.22360 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.003 |
RMSD bond angle | 0.730 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 2.747 |
Number of reflections | 25912 |
Completeness [%] | 99.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 296 | 18% PEG 3350, 5% tacsimate pH 6.5, 2% ethylene glycol, 100 mM PIPES pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 296K |