4Q0I
Deinococcus radiodurans BphP PAS-GAF D207A mutant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X29A |
Synchrotron site | NSLS |
Beamline | X29A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-12-11 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.07500 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 87.497, 51.327, 80.368 |
Unit cell angles | 90.00, 115.75, 90.00 |
Refinement procedure
Resolution | 36.193 - 1.744 |
R-factor | 0.163 |
Rwork | 0.161 |
R-free | 0.19510 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.015 |
RMSD bond angle | 1.623 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 36.500 |
High resolution limit [Å] | 1.744 |
Number of reflections | 32523 |
Completeness [%] | 99.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 296 | 19% isopropano, 19% PEG 3350, 5% glycerol, 100 mM Citric acid(NaOH) pH 5.6, VAPOR DIFFUSION, SITTING DROP, temperature 296K |