4Q0H
Deinococcus radiodurans BphP PAS-GAF
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-D |
Synchrotron site | APS |
Beamline | 23-ID-D |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-07-30 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.68880 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 89.536, 51.608, 80.633 |
Unit cell angles | 90.00, 116.32, 90.00 |
Refinement procedure
Resolution | 36.137 - 1.157 |
R-factor | 0.1312 |
Rwork | 0.131 |
R-free | 0.15710 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.009 |
RMSD bond angle | 1.328 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 1.157 |
Number of reflections | 111918 |
Completeness [%] | 98.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 296 | 10% PEG 3350, 19% isopropanol, 5% glycerol, 100 mM citric acid(NaOH) ph 5.6, VAPOR DIFFUSION, SITTING DROP, temperature 296K |