4Q0G
Crystal structure of beta subunit of acyl-CoA carboxylase AccD1 from Mycobacterium tuberculosis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2011-06-05 |
| Detector | RAYONIX MX300HE |
| Wavelength(s) | 0.97949 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 142.571, 221.253, 167.835 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.810 - 2.310 |
| R-factor | 0.19044 |
| Rwork | 0.189 |
| R-free | 0.21325 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3u9r |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.015 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.810 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.157 | 0.817 |
| Number of reflections | 115910 | |
| <I/σ(I)> | 12.93 | 2.66 |
| Completeness [%] | 99.7 | 100 |
| Redundancy | 5.8 | 5.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 0.1 M sodium HEPES pH 7.5 and 30% PEG300, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
