4PZL
The crystal structure of adenylate kinase from Francisella tularensis subsp. tularensis SCHU S4
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-03-10 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97924, 0.97938 |
| Spacegroup name | P 32 |
| Unit cell lengths | 65.022, 65.022, 214.732 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 34.148 - 2.100 |
| R-factor | 0.1936 |
| Rwork | 0.191 |
| R-free | 0.23680 |
| Structure solution method | MAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.089 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | SHELXD |
| Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.500 | 2.140 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.071 | 0.664 |
| Number of reflections | 59141 | |
| <I/σ(I)> | 30.1 | 1.7 |
| Completeness [%] | 99.6 | 100 |
| Redundancy | 3.8 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 289 | 0.2M CaCl2, 0.1M Tris, 20% (w/v) PEG4000., pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
