4PZJ
1.60 Angstrom resolution crystal structure of a transcriptional regulator of the LysR family from Eggerthella lenta DSM 2243
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-D |
Synchrotron site | APS |
Beamline | 21-ID-D |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-03-20 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.97875 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 31.623, 31.623, 132.340 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 28.550 - 1.600 |
R-factor | 0.19072 |
Rwork | 0.190 |
R-free | 0.21322 |
Structure solution method | SAD |
RMSD bond length | 0.015 |
RMSD bond angle | 2.141 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | HKL-3000 |
Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.630 |
High resolution limit [Å] | 1.600 | 1.600 |
Rmerge | 0.077 | 0.496 |
Number of reflections | 9452 | |
<I/σ(I)> | 51.2 | 3.42 |
Completeness [%] | 98.1 | 85.7 |
Redundancy | 12.2 | 7.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 295 | protein at 1.3 mg/mL in 10 mM Tris-HCl pH 8.3, 500 mM NaCl, 5 mM BME. crystallization: The Classics II Suite (A12 (#12): 100 mM Tris pH 8.5, 3 M NaCl), VAPOR DIFFUSION, SITTING DROP, temperature 295K. Cryo condition: 25% (final) sucrose. |