4PYR
Structure of a putative branched-chain amino acid ABC transporter from Chromobacterium violaceum ATCC 12472
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-F |
| Synchrotron site | APS |
| Beamline | 21-ID-F |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-02-03 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.97872 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 41.182, 72.332, 45.146 |
| Unit cell angles | 90.00, 95.13, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.450 |
| R-factor | 0.18793 |
| Rwork | 0.186 |
| R-free | 0.23289 |
| Structure solution method | SAD |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.723 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.480 |
| High resolution limit [Å] | 1.450 | 1.450 |
| Rmerge | 0.070 | 0.520 |
| Number of reflections | 46422 | |
| <I/σ(I)> | 34.3 | 2.2 |
| Completeness [%] | 99.6 | 100 |
| Redundancy | 3.7 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 0.1 M Hepes, 25% PEG3350, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
