4PYP

Crystal structure of the human glucose transporter GLUT1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2014-03-02
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97906
Spacegroup name	C 1 2 1
Unit cell lengths	120.509, 101.996, 65.604
Unit cell angles	90.00, 101.27, 90.00

Refinement procedure

Resolution	32.674 - 3.166
R-factor	0.2343
Rwork	0.232
R-free	0.27760
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4gc0
RMSD bond length	0.009
RMSD bond angle	1.252
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.8.4_1496))

Data quality characteristics

	Overall
Low resolution limit [Å]	40.000
High resolution limit [Å]	3.160
Number of reflections	12780
Completeness [%]	99.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	277	30% PEG400, 0.1M MgCl2, 0.1M MES, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K