4PY7
Crystal Structure of Fab 3.1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 75 |
| Detector technology | CCD |
| Collection date | 2011-10-12 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.71941 |
| Spacegroup name | P 43 |
| Unit cell lengths | 73.842, 73.842, 207.954 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.700 |
| R-factor | 0.22998 |
| Rwork | 0.228 |
| R-free | 0.27270 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.498 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.750 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Number of reflections | 30297 | |
| Completeness [%] | 98.2 | 98.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 295 | 0.2 M calcium acetate, 10% PEG8000, 100 mM Tris, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
