4PXG
Crystal Structure of TypeII restriction Enzyme Sau3AI
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BSRF BEAMLINE 3W1A |
Synchrotron site | BSRF |
Beamline | 3W1A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-07-07 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 0.9789 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 69.648, 197.673, 191.145 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 2.450 |
R-factor | 0.22576 |
Rwork | 0.224 |
R-free | 0.26331 |
Structure solution method | SAD |
RMSD bond length | 0.012 |
RMSD bond angle | 1.361 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | SOLVE |
Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 54.140 | 54.140 | 2.680 |
High resolution limit [Å] | 2.450 | 6.000 | 2.450 |
Rmerge | 0.062 | 0.036 | 0.331 |
Number of reflections | 47905 | ||
<I/σ(I)> | 70.9 | 3.5 | |
Completeness [%] | 8.6 | 7.7 | 24.3 |
Redundancy | 11.6 | 12.9 | 4.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 277 | 16% polyethylene glycol 8000, 1M sodium acetate, 0.2M calcium chloride, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 277 | 16% polyethylene glycol 8000, 1M sodium acetate, 0.2M calcium chloride, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 277K |