4PX7
Crystal structure of lipid phosphatase E. coli PgpB
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-1A |
| Synchrotron site | Photon Factory |
| Beamline | BL-1A |
| Temperature [K] | 200 |
| Detector technology | PIXEL |
| Collection date | 2013-10-26 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9788 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 36.715, 73.667, 131.200 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 32.860 - 3.200 |
| R-factor | 0.2715 |
| Rwork | 0.270 |
| R-free | 0.30230 |
| Structure solution method | SAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.258 |
| Data reduction software | DENZO |
| Data scaling software | SCALA |
| Phasing software | SOLVE |
| Refinement software | PHENIX ((phenix.refine: 1.8_1069)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.860 | 3.310 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Rmerge | 0.129 | 0.717 |
| Number of reflections | 6299 | |
| <I/σ(I)> | 3.7 | |
| Completeness [%] | 99.0 | 90.5 |
| Redundancy | 11.8 | 10.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 289 | 20mM CaCl2, 10mM MgSO4, 20mM MES (pH 6.5), 7.7% (w/v) polyethylene glycol 1500, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
