4PWU
Crystal structure of a modulator protein MzrA (KPN_03524) from Klebsiella pneumoniae subsp. pneumoniae MGH 78578 at 2.45 A resolution
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL14-1 |
Synchrotron site | SSRL |
Beamline | BL14-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-12-17 |
Detector | MARMOSAIC 325 mm CCD |
Wavelength(s) | 0.97934 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 61.770, 74.380, 74.200 |
Unit cell angles | 90.00, 99.26, 90.00 |
Refinement procedure
Resolution | 37.573 - 2.450 |
R-factor | 0.2013 |
Rwork | 0.199 |
R-free | 0.25010 |
Structure solution method | MAD |
RMSD bond length | 0.010 |
RMSD bond angle | 0.840 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | SHELX |
Refinement software | BUSTER-TNT (2.10.0) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 37.573 | 37.570 | 2.510 |
High resolution limit [Å] | 2.450 | 10.960 | 2.450 |
Rmerge | 0.075 | 0.038 | 1.409 |
Number of reflections | 12229 | 145 | 894 |
<I/σ(I)> | 15.84 | 42.2 | 1.84 |
Completeness [%] | 99.6 | 95.4 | 99.9 |
Redundancy | 7.56 | 7.67 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.83 | 293 | 1.40M sodium acetate, 0.1M sodium cacodylate pH 5.83, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
1 | VAPOR DIFFUSION, SITTING DROP | 5.83 | 293 | 1.40M sodium acetate, 0.1M sodium cacodylate pH 5.83, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 293K |