4PWM
Crystal structure of Dickerson Drew Dodecamer with 5-carboxycytosine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-12-05 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97920 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 24.250, 41.340, 66.410 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.890 - 1.950 |
| R-factor | 0.22492 |
| Rwork | 0.221 |
| R-free | 0.26721 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ID 436D |
| RMSD bond length | 0.009 |
| RMSD bond angle | 2.209 |
| Data reduction software | XDS (package) |
| Data scaling software | XDS (package) |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 26.000 | 2.000 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.045 | 0.620 |
| Number of reflections | 5113 | |
| <I/σ(I)> | 16.26 | 2.8 |
| Completeness [%] | 97.5 | 99.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 291 | 40 mM Na Cacodylate, 12 mM Spermine Tetrahydrochloride, 80 mM SrCl2, 10% MPD , pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
