4PWE
Crystal structure of V30M mutant human transthyretin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-10-27 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 43.558, 85.461, 64.528 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 36.100 - 1.400 |
| R-factor | 0.20449 |
| Rwork | 0.203 |
| R-free | 0.22816 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4n85 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.347 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.100 | 1.420 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Rmerge | 0.046 | 0.446 |
| Number of reflections | 43714 | |
| <I/σ(I)> | 24.6 | 2.4 |
| Completeness [%] | 94.6 | 82.3 |
| Redundancy | 5.2 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 30% PEG400, 0.1M HEPES, 0.4M CaCl2, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
