4PW9
Crystal structure of the electron-transfer complex formed between a sulfite dehydrogenase and a c-type cytochrome from Sinorhizobium meliloti
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
Synchrotron site | Australian Synchrotron |
Beamline | MX2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-07-15 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.954 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 109.583, 95.815, 49.906 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.560 - 2.490 |
R-factor | 0.214 |
Rwork | 0.211 |
R-free | 0.26000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4pw3 |
RMSD bond length | 0.012 |
RMSD bond angle | 1.412 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.590 |
High resolution limit [Å] | 2.490 | 2.500 |
Rmerge | 0.139 | 0.766 |
Number of reflections | 18883 | |
<I/σ(I)> | 5.8 | 2.1 |
Completeness [%] | 99.3 | 99.6 |
Redundancy | 3.4 | 3.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 0.2M SODIUM FORMATE, 0.1M BIS-TRIS PROPANE PH 7.5, 20% PEG 3350, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 298K |