4PW6
structure of UHRF2-SRA in complex with a 5hmC-containing DNA, complex II
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-06-27 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9788 |
| Spacegroup name | P 41 |
| Unit cell lengths | 115.368, 115.368, 41.049 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.674 - 3.789 |
| R-factor | 0.2365 |
| Rwork | 0.236 |
| R-free | 0.28650 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3oln |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.595 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.940 |
| High resolution limit [Å] | 3.789 | 3.789 |
| Rmerge | 0.129 | 0.514 |
| Number of reflections | 5480 | |
| <I/σ(I)> | 10.8 | 2.8 |
| Completeness [%] | 98.0 | 99.8 |
| Redundancy | 4 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 289 | 0.1 M Tris pH 8.0 and 8% PEG-8000, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
