4PW2
Crystal structure of D-glucuronyl C5 epimerase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-G |
| Synchrotron site | APS |
| Beamline | 21-ID-G |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-02-15 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.97856 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 66.379, 66.379, 337.571 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.380 - 1.900 |
| R-factor | 0.21189 |
| Rwork | 0.210 |
| R-free | 0.24424 |
| Structure solution method | SAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.124 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | SHELXS |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.101 | 1.040 |
| Number of reflections | 61140 | |
| <I/σ(I)> | 14.8 | 3.2 |
| Completeness [%] | 100.0 | 99.9 |
| Redundancy | 14 | 14.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 293 | 16% w/v PEG 3,350, 0.1 M sodium citrate tribasic dihydrate pH 5.6, and 2% v/v Tacsimate pH 5.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
