4PW2
Crystal structure of D-glucuronyl C5 epimerase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-G |
Synchrotron site | APS |
Beamline | 21-ID-G |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-02-15 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.97856 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 66.379, 66.379, 337.571 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.380 - 1.900 |
R-factor | 0.21189 |
Rwork | 0.210 |
R-free | 0.24424 |
Structure solution method | SAD |
RMSD bond length | 0.006 |
RMSD bond angle | 1.124 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | SHELXS |
Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.000 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.101 | 1.040 |
Number of reflections | 61140 | |
<I/σ(I)> | 14.8 | 3.2 |
Completeness [%] | 100.0 | 99.9 |
Redundancy | 14 | 14.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 293 | 16% w/v PEG 3,350, 0.1 M sodium citrate tribasic dihydrate pH 5.6, and 2% v/v Tacsimate pH 5.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |