4PW1
Crystal structure of a protein with unknown function (CLOLEP_02462) from Clostridium leptum DSM 753 at 2.10 A resolution
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL11-1 |
Synchrotron site | SSRL |
Beamline | BL11-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-11-26 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.91837,0.97959,0.97903 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 72.101, 72.101, 214.255 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.907 - 2.100 |
R-factor | 0.1653 |
Rwork | 0.164 |
R-free | 0.19700 |
Structure solution method | MAD |
RMSD bond length | 0.015 |
RMSD bond angle | 1.541 |
Data reduction software | MOSFLM |
Data scaling software | SCALA (3.3.20) |
Phasing software | SHELX |
Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 29.907 | 29.907 | 2.150 |
High resolution limit [Å] | 2.100 | 9.390 | 2.100 |
Rmerge | 0.046 | 0.285 | |
Total number of observations | 2293 | 13137 | |
Number of reflections | 33887 | ||
<I/σ(I)> | 14.5 | 10.5 | 2.7 |
Completeness [%] | 99.6 | 94.7 | 99.1 |
Redundancy | 5.9 | 5.1 | 5.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 9.33 | 277 | 0.20M lithium sulfate, 0.90M di-potassium hydrogen phosphate, 1.083M sodium dihydrogen phosphate, 0.1M Glycine pH 9.33, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 277K |