4PVL
X-ray structure of human transthyretin (TTR) at room temperature to 1.9A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 293 |
| Detector technology | CCD |
| Collection date | 2011-02-12 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9791 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 43.680, 86.260, 65.720 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.130 - 1.851 |
| R-factor | 0.1563 |
| Rwork | 0.152 |
| R-free | 0.20070 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ipe |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.282 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE: 1.7.3_914)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.130 | 1.950 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.093 | 0.420 |
| Number of reflections | 21718 | |
| <I/σ(I)> | 7.4 | 2.67 |
| Completeness [%] | 99.4 | 97.9 |
| Redundancy | 3.5 | 3.49 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.4 | 293 | 2.15M SODIUM MALONATE, 23MG/ML PROTEIN, 100UL DROP, pH 6.4, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
