4PV1
Cytochrome B6F structure from M. laminosus with the quinone analog inhibitor stigmatellin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-07-07 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 159.085, 159.085, 361.322 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 48.340 - 3.000 |
| R-factor | 0.217 |
| Rwork | 0.216 |
| R-free | 0.24700 |
| Structure solution method | RIGID BODY REFINEMENT AND RESTRAINED REFINEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.646 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | REFMAC |
| Refinement software | PHENIX ((PHENIX.REFINE: 1.8.4_1496)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 137.772 | 3.050 |
| High resolution limit [Å] | 2.997 | 3.000 |
| Number of reflections | 50303 | |
| <I/σ(I)> | 17.4 | 0.6 |
| Completeness [%] | 91.4 | 38 |
| Redundancy | 9.8 | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.3 | PH 8.3, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 277K |
