4PUV
URATE OXIDASE DI-AZIDE complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-1 |
Synchrotron site | ESRF |
Beamline | ID23-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-01-21 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.938 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 79.180, 94.920, 104.530 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 1.300 |
R-factor | 0.1762 |
Rwork | 0.176 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 4oqc |
RMSD bond length | 0.030 |
RMSD bond angle | 0.075 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | SHELXDE |
Refinement software | SHELXL-97 |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 1.340 |
High resolution limit [Å] | 1.300 | 1.300 |
Rmerge | 0.072 | 0.217 |
Number of reflections | 66686 | |
Completeness [%] | 98.8 | 99 |
Redundancy | 4.7 | 4.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | BATCH METHOD | 8.5 | 291 | MIXING TWO SOLUTIONS A & B: SOLUTION A: PROTEIN (20 MG/ML) TRIS ACETATE BUFFER 0.05 M PH=8.5. SOLUTION B: TRIS ACETATE BUFFER 0.05 M + SODIUM AZIDE 0.3M. CRYSTALS HARVESTED 2 MONTHS AFTER CRYSTALLIZATION (AGED CRYSTALS), BATCH METHOD, temperature 291K |