4PUI
BolA domain of SufE1 from Arabidopsis thaliana
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-03-05 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.979110 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 95.833, 31.634, 68.631 |
| Unit cell angles | 90.00, 116.83, 90.00 |
Refinement procedure
| Resolution | 46.182 - 1.698 |
| R-factor | 0.1924 |
| Rwork | 0.191 |
| R-free | 0.21320 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1v60 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.066 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((phenix.refine: 1.7.3_928)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.182 | 1.790 |
| High resolution limit [Å] | 1.698 | 1.698 |
| Rmerge | 0.032 | 0.357 |
| Number of reflections | 20573 | |
| <I/σ(I)> | 20.6 | 2.6 |
| Completeness [%] | 99.5 | 97.2 |
| Redundancy | 3.2 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Oil microbatch method | 6.5 | 278 | 12% PEG20000, 6% ethylene glycol, 0.02 M trimethylamine HCl and 0.1 M MES, pH 6.5, Oil microbatch method, temperature 278K |
