4PUG
BolA1 from Arabidopsis thaliana
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM30A |
Synchrotron site | ESRF |
Beamline | BM30A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-02-18 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.979733 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 30.088, 179.875, 66.818 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 44.969 - 1.998 |
R-factor | 0.2007 |
Rwork | 0.199 |
R-free | 0.22580 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4pui |
RMSD bond length | 0.003 |
RMSD bond angle | 0.849 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | PHENIX ((phenix.refine: 1.7.3_928)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 44.969 | 2.110 |
High resolution limit [Å] | 1.998 | 2.000 |
Rmerge | 0.102 | 0.351 |
Number of reflections | 12798 | |
<I/σ(I)> | 11.3 | 4.1 |
Completeness [%] | 99.8 | 98.9 |
Redundancy | 4.6 | 4.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Oil microbatch method | 278 | 18% PEG4000, 6% 2-propanol, 6% 2.5 hexanediol, 4% xylitol and 0.07 M sodium acetate, Oil microbatch method, temperature 278K |