4PUE
Extracellulr Xylanase from Geobacillus stearothermophilus: E159Q mutant, with xylotetraose in active site
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2013-09-10 |
| Detector | RIGAKU RAXIS HTC |
| Wavelength(s) | 1.54 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 122.403, 61.976, 89.486 |
| Unit cell angles | 90.00, 119.43, 90.00 |
Refinement procedure
| Resolution | 36.470 - 2.200 |
| R-factor | 0.17413 |
| Rwork | 0.172 |
| R-free | 0.21384 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1r86 |
| RMSD bond length | 0.019 |
| RMSD bond angle | 2.088 |
| Data reduction software | HKL-3000 |
| Data scaling software | SCALA |
| Phasing software | REFMAC (5.7.0032) |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.260 |
| High resolution limit [Å] | 2.200 | 2.203 |
| Number of reflections | 27073 | |
| Completeness [%] | 96.0 | 89.31 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.7 | 293 | 16% PEG 4000, 0.1M MES, 10mM Zinc chloride, pH 6.7, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
