4PUC

Crystal structure of a SusD homolog (BACUNI_02643) from Bacteroides uniformis ATCC 8492 at 2.00 A resolution

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL11-1
Synchrotron site	SSRL
Beamline	BL11-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-11-26
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.91162,0.97959,0.97889
Spacegroup name	P 21 21 21
Unit cell lengths	66.540, 112.190, 150.080
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	89.858 - 2.000
R-factor	0.1697
Rwork	0.168
R-free	0.19980
Structure solution method	MAD
RMSD bond length	0.006
RMSD bond angle	1.038
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	SHELX
Refinement software	REFMAC (5.7.0032)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	89.858		2.050
High resolution limit [Å]	2.000	8.940	2.000
Rmerge	0.061	0.015	0.547
Number of reflections	59887	1528	6645
<I/σ(I)>	9.89	36.4	1.4
Completeness [%]	75.1	92.3	61.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	277	0.2M zinc acetate, 10.0% polyethylene glycol 8000, 0.1M MES pH 6.0, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 277K