4PTB
Crystal structure of human SP100 PHD-Bromodomain in the free state
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-12-02 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.282 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 127.520, 45.183, 83.188 |
| Unit cell angles | 90.00, 102.21, 90.00 |
Refinement procedure
| Resolution | 27.720 - 1.600 |
| R-factor | 0.17402 |
| Rwork | 0.173 |
| R-free | 0.19229 |
| Structure solution method | SAD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.392 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | SHELXS |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 28.370 | 28.370 | 1.690 |
| High resolution limit [Å] | 1.600 | 4.730 | 1.600 |
| Rmerge | 0.045 | 0.041 | 0.291 |
| Number of reflections | 58158 | ||
| <I/σ(I)> | 23.3 | 30.74 | 4.65 |
| Completeness [%] | 100.0 | 100 | 100 |
| Redundancy | 6.7 | 3.32 | 3.45 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 277 | 22% PEG 20000, 0.1M MES pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
