4PSN
Crystal structure of apeThermo-DBP-RP2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.2 |
Synchrotron site | BESSY |
Beamline | 14.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-05-07 |
Detector | RAYONIX MX-225 |
Wavelength(s) | 0.9800 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 61.240, 108.640, 154.880 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 32.727 - 2.050 |
R-factor | 0.1811 |
Rwork | 0.179 |
R-free | 0.21590 |
Structure solution method | SAD |
RMSD bond length | 0.008 |
RMSD bond angle | 1.082 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | HKL2Map |
Refinement software | PHENIX ((phenix.refine: 1.6.4_486)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.100 |
High resolution limit [Å] | 2.050 | 2.050 |
Completeness [%] | 99.9 | 100 |
Redundancy | 7.4 | 5.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | 0.1 M imidazole, pH 8.0, 0.4 M NaH2PO4, 1.6 M K2HPO4, 0.2 M NaCl and 0.25 M glycine, VAPOR DIFFUSION, SITTING DROP, temperature 293K |