4PSK
Mycobacterium tuberculosis RecA phosphate bound low temperature structure I-LT
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2008-04-01 |
Detector | MAR scanner 345 mm plate |
Wavelength(s) | 1.54179 |
Spacegroup name | P 61 |
Unit cell lengths | 107.120, 107.120, 71.070 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 29.610 - 2.800 |
R-factor | 0.19816 |
Rwork | 0.192 |
R-free | 0.25414 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1g19 |
RMSD bond length | 0.006 |
RMSD bond angle | 1.111 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 38.870 | 2.950 |
High resolution limit [Å] | 2.800 | 2.800 |
Rmerge | 0.106 | 0.510 |
Number of reflections | 11506 | |
<I/σ(I)> | 8.6 | 2 |
Redundancy | 3 | 2.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.8 | 298 | 30% PEG 3350, 0.2M AMMONIUM ACETATE, 0.1M SODIUM CITRATE, pH 5.8, VAPOR DIFFUSION, HANGING DROP, temperature 298K |