4PSI

PIH1D1/phospho-Tel2 complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I02
Synchrotron site	Diamond
Beamline	I02
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-06-01
Detector	DECTRIS PILATUS 2M
Wavelength(s)	0.9790
Spacegroup name	P 21 21 21
Unit cell lengths	49.600, 81.840, 83.990
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.843 - 2.450
R-factor	0.2413
Rwork	0.240
R-free	0.26840
Structure solution method	SAD
RMSD bond length	0.003
RMSD bond angle	0.802
Refinement software	PHENIX ((phenix.refine: 1.8.2_1309))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.550
High resolution limit [Å]	2.450	2.450
Number of reflections	13117
Completeness [%]	99.9	99.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	291	Crystals grew from a 8 mg/ml solution of complex in 20 mM Tris pH 8.0, 150 mM NaCl, 0.5 mM TCEP equilibrated well solution containing 32 % w/v PEG4000, 100 mM sodium acetate, 100 mM Tris pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 291K