4PSB
Crystal Structure of Phytohormone Binding Protein from Vigna radiata in complex with gibberellic acid (GA3)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2) |
Synchrotron site | PETRA III, EMBL c/o DESY |
Beamline | P14 (MX2) |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-09-12 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97551 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 34.380, 54.290, 71.090 |
Unit cell angles | 90.00, 98.70, 90.00 |
Refinement procedure
Resolution | 35.140 - 1.420 |
R-factor | 0.1543 |
Rwork | 0.152 |
R-free | 0.20390 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2flh |
RMSD bond length | 0.019 |
RMSD bond angle | 1.911 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 35.140 | 35.140 | 1.460 |
High resolution limit [Å] | 1.420 | 6.350 | 1.420 |
Rmerge | 0.079 | 0.043 | 0.520 |
Number of reflections | 24350 | ||
<I/σ(I)> | 9.2 | 20.51 | 1.98 |
Completeness [%] | 99.5 | 99.7 | 99.3 |
Redundancy | 4.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4 | 292 | 0.1 M MMT buffer pH 4.0, 25% PEG 1500, VAPOR DIFFUSION, HANGING DROP, temperature 292K |