4PS8
Structure of PI3K gamma in complex with N-[6-(5,6-dimethoxypyridin-3-yl)-1,3-benzothiazol-2-yl]acetamide
Experimental procedure
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.1 |
Synchrotron site | ALS |
Beamline | 5.0.1 |
Temperature [K] | 98 |
Detector technology | CCD |
Collection date | 2005-06-12 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 143.570, 67.684, 106.542 |
Unit cell angles | 90.00, 96.21, 90.00 |
Refinement procedure
Resolution | 105.920 - 2.990 |
R-factor | 0.19699 |
Rwork | 0.193 |
R-free | 0.27924 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.014 |
RMSD bond angle | 1.693 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | AMoRE |
Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 105.920 | |
High resolution limit [Å] | 2.980 | 2.980 |
Rmerge | 0.059 | |
Number of reflections | 17868 | |
Completeness [%] | 92.0 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 298 | 100 mM Tris, pH 8.5, 25% w/v PEG3350, 200 mM lithium sulfate, 10 mM DTT, 10 mM EDTA, VAPOR DIFFUSION, HANGING DROP, temperature 298K |