4PS2
Structure of the C-terminal fragment (87-165) of E.coli EAEC TssB molecule
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-04-29 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1.28 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 52.640, 69.150, 45.880 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 41.880 - 2.000 |
| R-factor | 0.1847 |
| Rwork | 0.181 |
| R-free | 0.21680 |
| Structure solution method | SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 0.970 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | SHELXS |
| Refinement software | BUSTER (2.11.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.000 | 2.120 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.123 | 0.990 |
| Number of reflections | 5921 | |
| <I/σ(I)> | 11.3 | 2 |
| Completeness [%] | 99.5 | 97.4 |
| Redundancy | 5.2 | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | Zinc acetate 0.2 M, PEG3350 20%, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
