4PRW
Xylanase T6 (XT6) from Geobacillus Stearothermophilus in complex with xylohexaose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2013-09-10 |
| Detector | RIGAKU RAXIS HTC |
| Wavelength(s) | 1.54 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 121.800, 61.670, 89.350 |
| Unit cell angles | 90.00, 119.38, 90.00 |
Refinement procedure
| Resolution | 43.070 - 1.800 |
| R-factor | 0.15341 |
| Rwork | 0.151 |
| R-free | 0.19413 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1r86 |
| RMSD bond length | 0.021 |
| RMSD bond angle | 2.158 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | REFMAC (5.7.0032) |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.070 | 1.900 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Number of reflections | 45528 | |
| Completeness [%] | 89.4 | 49 |
| Redundancy | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.7 | 293 | 30% PEG 4K, 0.01M ZnCl2, 0.1M MES buffer, pH 6.7, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
