4PRS
Structure of apo ArgBP from T. maritima
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X12 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X12 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.9796, 0.9894, 0.9537 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 116.811, 51.966, 98.997 |
| Unit cell angles | 90.00, 122.81, 90.00 |
Refinement procedure
| Resolution | 14.820 - 1.470 |
| R-factor | 0.15254 |
| Rwork | 0.150 |
| R-free | 0.20405 |
| Structure solution method | MAD |
| RMSD bond length | 0.026 |
| RMSD bond angle | 1.987 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.5.0110) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.520 |
| High resolution limit [Å] | 1.470 | 1.470 |
| Number of reflections | 85761 | |
| Completeness [%] | 98.7 | 87.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 293 | 25%(w/v) PEG 3350, 0.1M Sodium Acetate trihydrate, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
